Identification and In Situ Distribution of a Fungal Gene Marker: The Mating Type Genes of the Black Truffle.
Identifieur interne : 000158 ( Main/Exploration ); précédent : 000157; suivant : 000159Identification and In Situ Distribution of a Fungal Gene Marker: The Mating Type Genes of the Black Truffle.
Auteurs : Herminia De La Varga [France] ; Claude Murat [France]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2016.
Descripteurs français
- KwdFr :
- Ascomycota (croissance et développement), Ascomycota (génétique), Gènes fongiques du type conjugant (génétique), Humains (MeSH), Microbiologie du sol (MeSH), Protéines fongiques (génétique), Protéines fongiques (isolement et purification), Réaction de polymérisation en chaîne (méthodes), Écosystème (MeSH).
- MESH :
- croissance et développement : Ascomycota.
- génétique : Ascomycota, Gènes fongiques du type conjugant, Protéines fongiques.
- isolement et purification : Protéines fongiques.
- méthodes : Réaction de polymérisation en chaîne.
- Humains, Microbiologie du sol, Écosystème.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Fungal Proteins.
- genetics : Ascomycota, Genes, Mating Type, Fungal.
- growth & development : Ascomycota.
- chemical , isolation & purification : Fungal Proteins.
- methods : Polymerase Chain Reaction.
- Ecosystem, Humans, Soil Microbiology.
Abstract
Truffles are ectomycorrhizal fungi harvested mainly in human managed agroforestry ecosystems. Truffle production in truffle orchards faces two important bottlenecks or challenges: the initiation of the sexual reproduction and the growth of the ascocarps during several months. The black Périgord truffle, Tuber melanosporum, is a heterothallic species and the mating type genes (MAT1-1 and M1T1-2) have been characterized. In this context, the unraveling of the T. melanosporum mating type strains distribution in truffle orchards is a critical starting point to provide new insights into its sexual reproduction. The aim of this chapter is to present the protocol used to characterize the T. melanosporum mating type present in a truffle orchard from ascocarps, hazel mycorrhizal root tips, and/or soil samples, by polymerase chain reactions using specific primers for those genes, but it can be adapted for other fungal species.
DOI: 10.1007/978-1-4939-3369-3_8
PubMed: 26791501
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Truffle production in truffle orchards faces two important bottlenecks or challenges: the initiation of the sexual reproduction and the growth of the ascocarps during several months. The black Périgord truffle, Tuber melanosporum, is a heterothallic species and the mating type genes (MAT1-1 and M1T1-2) have been characterized. In this context, the unraveling of the T. melanosporum mating type strains distribution in truffle orchards is a critical starting point to provide new insights into its sexual reproduction. The aim of this chapter is to present the protocol used to characterize the T. melanosporum mating type present in a truffle orchard from ascocarps, hazel mycorrhizal root tips, and/or soil samples, by polymerase chain reactions using specific primers for those genes, but it can be adapted for other fungal species. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26791501</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>14</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1399</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Identification and In Situ Distribution of a Fungal Gene Marker: The Mating Type Genes of the Black Truffle.</ArticleTitle><Pagination><MedlinePgn>141-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-3369-3_8</ELocationID><Abstract><AbstractText>Truffles are ectomycorrhizal fungi harvested mainly in human managed agroforestry ecosystems. Truffle production in truffle orchards faces two important bottlenecks or challenges: the initiation of the sexual reproduction and the growth of the ascocarps during several months. The black Périgord truffle, Tuber melanosporum, is a heterothallic species and the mating type genes (MAT1-1 and M1T1-2) have been characterized. In this context, the unraveling of the T. melanosporum mating type strains distribution in truffle orchards is a critical starting point to provide new insights into its sexual reproduction. The aim of this chapter is to present the protocol used to characterize the T. melanosporum mating type present in a truffle orchard from ascocarps, hazel mycorrhizal root tips, and/or soil samples, by polymerase chain reactions using specific primers for those genes, but it can be adapted for other fungal species. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>De la Varga</LastName><ForeName>Herminia</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>UMR1136 INRA/University of Lorraine "Tree-Microbe Interactions" (IAM), Labex ARBRE, Champenoux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Murat</LastName><ForeName>Claude</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>UMR1136 INRA/University of Lorraine "Tree-Microbe Interactions" (IAM), Labex ARBRE, Champenoux, France. claude.murat@nancy.inra.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017753" MajorTopicYN="N">Ecosystem</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049770" MajorTopicYN="N">Genes, Mating Type, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Ascocarps</Keyword><Keyword MajorTopicYN="N">DNA</Keyword><Keyword MajorTopicYN="N">Ectomycorrhiza</Keyword><Keyword MajorTopicYN="N">Mating type genes</Keyword><Keyword MajorTopicYN="N">Polymerase chain reaction (PCR)</Keyword><Keyword MajorTopicYN="N">Soil</Keyword><Keyword MajorTopicYN="N">Tuber melanosporum</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>1</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>1</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26791501</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-3369-3_8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Champenoux</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="De La Varga, Herminia" sort="De La Varga, Herminia" uniqKey="De La Varga H" first="Herminia" last="De La Varga">Herminia De La Varga</name></region><name sortKey="Murat, Claude" sort="Murat, Claude" uniqKey="Murat C" first="Claude" last="Murat">Claude Murat</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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